A free-standing electrode was developed based on carbon cloth-supported Fe doped polydopamine derived carbon (Fe/PDA-C/CC) for detection of dopamine (DA) and uric acid (UA) simultaneously. First, dopamine was self-polymerized on the surface of carbon cloth to obtain the polydopamine coatings. Then, Fe3+ was introduced by the formation of coordinate bond with the hydroxyl functional group in the polydopamine layer. After calcination, a flexible and free-standing electrode was obtained. The sensing performance and mechanism of the Fe/PDA-C/CC sensor was investigated and discussed in detail. The experiment results demonstrated that the Fe/PDA-C/CC could simultaneous detection of DA and UA with a wide detection range of 0.5-300 μM and 0.5-400 μM, with low detection limits of 0.041 μM and 0.012 μM, respectively. Meanwhile, the Fe/PDA-C/CC possessed excellent anti-interference, repeatability, stability, as well as accuracy in real samples. Overall, this study provided a facile and effect approach for simultaneous detection of UA and DA.